Part:BBa_K4475003:Design

CTB1 Constitutive Strong Protein Generator Yeast

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4213
  Illegal BamHI site found at 4122
  Illegal BamHI site found at 6295
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 6802
  Illegal AgeI site found at 4876
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The native coding sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.

Source

Genome of Cercospora Beticola